Natural remedies have been integral to alternative medicine for thousands of years, with numerous drugs now derived from plants and animals. Pisum sativum, known as "Motorsuti" in Bangladesh, is one such medicinal plant recognized for its healing properties. This study aimed to investigate the phytochemical composition of methanol extract from P. sativum seeds grown in Bangladesh and to evaluate its antibacterial and antioxidant activities using validated experimental methods. The antioxidant activity was assessed in vitro using the 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, while the antibacterial efficacy was determined via the well diffusion method against various pathogenic bacterial strains. Phytochemical screening of the P. sativum seed extract revealed the presence of proteins, terpenoids, glycosides, alkaloids, carbohydrates, flavonoids, saponins, quinine, gum, and alkaloids. The DPPH radical scavenging activity of the methanol extract was measured as the percentage inhibition of DPPH at different extract doses. The extract demonstrated significant free radical scavenging activity, with an IC50 value of 178.65 µg/ml, compared to 60.19 µg/ml for the standard antioxidant ascorbic acid. The methanol extract also exhibited notable antibacterial activity against some gram-negative bacteria, including Pseudomonas spp., Citrobacter spp., and Enterobacter spp., with zones of inhibition measuring 11.50 mm, 15 mm, and 24 mm, respectively. These results suggest that the methanol extract of P. sativum seeds contains valuable secondary metabolites with potent antibacterial and antioxidant properties, highlighting its potential nutritional and pharmacological benefits.
Keywords: Antioxidant Assay, DPPH, Pisum sativum, Methanol, phytochemicals, free radicals, antibacterial agents.
31-Aug-2024
