Natural remedies have been used in alternative medicine for thousands of years, and now an astonishing variety of drugs have been isolated from plants and animals. Pisum sativum, also known as "Motorsuti" in Bangladesh, is one of the various medicinal plants that have the ability to heal a variety of ailments. The current study was carry out to screen the phytochemical properties of methanol extract of P. sativum plant seeds cultivated in Bangladesh as well as to study the antibacterial and antioxidant efficacyusing several validated experimental methods. In vitro antioxidant activity was evaluated using the 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay, while antibacterial activity was evaluated using the well diffusion method against a panel of pathogenic bacterial strains. Screening tests of the P. sativum seed extract demonstrated the presence of proteins, terpenoids, glycosides, alkaloids, carbohydrates, flavonoids, saponins, quinine, gum, and alkaloids.The DPPH radical scavenging capabilities of a pea seed methanol extract were evaluated as the % inhibition of DPPH at different doses of the extract. The methanol extract of Pisum sativum seeds was reported for considerable free radical scavenging activity. The extraction's IC50 value was 178.65 g/ml, compared to the standard anti-oxidant ascorbic acid's IC50 of 60.19 g/ml. The methanol extract of P. sativum seeds has also shown significant antibacterial activity against a subset of gram-negative bacteria in the current investigation namely Pseudomonas spp., Citrobacter spp., and Enterobacter spp. (zone of inhibition around 11.50 mm, 15 mm and 24 mm respectively).These findings, therefore, imply that the test substance is a herb with certain nutritionally and pharmacologically significant secondary metabolites that have good antibacterial and antioxidant properties.
Keywords: Antioxidant Assay, DPPH, Pisum sativum, Methanol, phytochemicals, free radicals, antibacterial agents.
30-Jun-2023
