The present study was conducted to investigate the protein denaturation, membrane stabilizing and cytotoxic activities of ethanolextract of Ficus hispida L fruits. Preliminary phytochemical screenings of the crude extractives demonstrated the presence of carbohydrates, glycosides, tannins, terpenoids, saponins, flavonoids, and steroids. In protein denaturation test, the extracts inhibited protein denaturationby9.56% at 600µg/ml, 5.03%at 300µg/ml and by 2.00%at 150µg/ml. The membrane stabilizing activity was determined using hypotonic solution induced haemolysis of human red blood cells. In the membranestabilizing assay, the crude ethanol extractexhibited inhibition of haemolysis of human RBCs by 29.59% at600µg/ml, 17.16% at 300µg/ml and by 8.28% at 150µg/ml.The brine shrimplethality bioassay is a rapid and widely used method that indicates cytotoxicity, anticancer, antiviral, pesticidal, insecticidal activities of the bioactive metabolites. In the cytotoxicity screening, the ethanol extract showed that the degree of lethality wasdirectly proportional to the concentration (LC50 165.96µg/ml) of the crudeextract.
Key words: Ficus hispidaL. fruits, Proteindenaturation, Membrane stabilizing, cytotoxic activity, Ethanol extract.
Nov 07 2024 , 10:52 AM
To the kyau.edu.bd owner, Your posts are always well presented.
28-Feb-2025
