Background: Different Parts of the perennial herb Operculina turpethum (L.J Silva Manso enjoys a variety of uses in ethnobotanical and ethnomedicinal practicein Bangladesh and demands biological investigations to reveal its therapeutic potentiality. In the present study, a number of phytochemical and pharmacological investigations were done on the ethanolic extract of Operculina turpethum roots to explore its antioxidant, cytotoxic and anthelmintic potential.
Methods: In vitro antioxidant activity was evaluated byDPPH free radicalscavenging assay whereastotal phenolic content (TPCJ was measured by using Folin Ciocalteu's reagent. Cytotoxic activity was determined by brine shrimp lethality bioassay while anthelmintic activity was tested on parasite Paramphistomum cervi.Results: The Phytochemical investigation revealed the presence of alkaloids, flavonoids, tannins, glycosides, phenolic compounds, gums etc. The extract showed free radical scavenging activity with IC50 value of 46.80µg/ml and standardascorbic acid content (TPCJwas found to be 33•87 mg GAE lg of dried plant material. The LC50 against brine shrimp nauplii was 56.76 µg/ml for Operculina turpethum root extract whereas 0.514 µg/ml for vincristine sulphate. In anthelmintic test, the extract showed significant and dose-dependent decreasein paralysis time and death time of parasites.
Conclusion: The results demonstrated that the ethanolic extract of the plant has potential antioxidant, cytotoxic and anthelmintic activity.
Keywords: Operculina, Antioxidant, Cytotoxic, Anthelmintic
28-Feb-2025
